3DExo+™ Matrix with Growth Factor (12B Exosomes Vial)

3DExo+™ is a next-generation cosmetic-grade exosome matrix derived from a proprietary 3D-cultured mesenchymal stem cell (MSC) secretome process. This advanced formulation leverages three-dimensional expansion of Wharton’s Jelly MSCs to produce a highly concentrated, cell-free solution rich in extracellular vesicles, peptides, and growth factors.

The 3DExo+™ platform is designed for topical cosmetic and wellness support applications, offering a more physiologically relevant and bioactive profile than traditional 2D-cultured exosome preparations.

Key Features:

 • 3D-cultured MSC secretome with high-density exosome content
 • Cell-free, non-viable, cosmetic-use only matrix
 • Xeno-free, serum-free, and human-derived manufacturing process
 • Produced in FDA-registered and ISO-compliant facilities
 • Optimized for skin rejuvenation, appearance enhancement, and wellness protocols
 • Not for use in the treatment of disease or medical conditions

Thawing Cells: Step-by-Step Procedure

1. Preparation of Warm Water
Warm sterile water in a 37°C water bath. Ensure that the water bath is calibrated correctly to maintain this temperature throughout the thawing process.

2. Surface Sterilization
Before handling the cell vial, wipe the outside with 70% ethanol or isopropanol to minimize contamination risks. This step is crucial for maintaining aseptic conditions.

3. Pressure Relief in Vial
In a biosafety cabinet, carefully twist the cap of the vial a quarter-turn to relieve any internal pressure that may have built up during freezing. Retighten the cap to prevent contamination.

4. Thawing Cells
- Place the vial in the 37°C water bath and gently shake it to facilitate thawing. Do not submerge the vial completely; only the lower part should be in contact with the warm water.
Monitor closely and remove the vial when only a small frozen cell pellet remains visible
(Thaw for NOT LONGER than 1 minute – to ice ball stage). Handle gently and completely avoid vortexing or shaking vigorously, as this will likely damage cells.

5. Second Surface Sterilization
Again, wipe the outside of the vial with 70% ethanol or isopropanol after thawing to ensure that any potential contaminants are removed before opening.

6. Dilution of Cells
Dilute the thawed cells by adding an equal volume of warmed culture medium (1:10 dilution). This step helps to reduce cellular stress and promotes recovery.

FDA Compliance Information

•  Cosmetic Classification: This product is a cosmetic under the Federal Food, Drug, and Cosmetic Act (21 U.S.C. § 301 et seq.) and is not a drug, biologic, or medical device. It is intended to enhance skin appearance (e.g., hydration, smoothness) and does not treat medical conditions or alter skin structure/function.
•  Labeling: Ingredients are listed per INCI standards (21 CFR 701).
•  Safety: Manufactured under cosmetic Good Manufacturing Practices (GMP, ISO 22716-aligned). Testing confirms sterility (no growth), no mycoplasma, and low endotoxin (<0.005 EU/mL).

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