⁠Mesenchymal Stem Cells (hUCT-MSC)

Introducing our premium Human Umbilical Cord Tissue-Derived Mesenchymal Stem Cells (hUCT-MSC), a cutting-edge solution for advanced regenerative medicine and research applications. Sourced from ethically donated umbilical cord tissue, these high-quality stem cells offer exceptional viability, purity, and potency, making them ideal for therapeutic development, tissue engineering, and scientific exploration.

Key Features

• High Purity and Viability: Our hUCT-MSCs are rigorously tested to ensure over 95% viability and purity, providing consistent and reliable performance in your experiments or clinical applications.

• Ethical Sourcing: Derived from healthy, consented donors, our stem cells comply with strict ethical and regulatory standards, ensuring peace of mind for researchers and clinicians.

• Multipotent Differentiation: These MSCs exhibit robust differentiation potential into adipocytes, osteocytes, and chondrocytes, supporting diverse regenerative applications.

• Cryopreserved for Convenience: Supplied in cryovials with a proprietary freezing medium, our hUCT-MSCs maintain optimal functionality upon thawing, streamlining your workflow.

• 5% DMSO Solution per vial - this prevents ice crystal formation in cells, maintains membrane integrity post-thaw, and balances efficacy & safety.

Applications

hUCT-MSCs are versatile tools for both research and clinical settings:

• Regenerative Medicine: Support tissue repair and regeneration in conditions like osteoarthritis, cardiovascular diseases, and wound healing.

• Immunomodulation Studies: Leverage their immunosuppressive properties to explore treatments for autoimmune disorders and graft-versus-host disease.

• Tissue Engineering: Use hUCT-MSCs to develop scaffolds for bone, cartilage, or soft tissue reconstruction.

• Drug Discovery: Employ these cells in high-throughput screening for novel therapeutics targeting inflammatory or degenerative diseases.

Why Choose Our hUCT-MSCs?

Our hUCT-MSCs stand out due to their superior quality and consistent performance. Manufactured in a cGMP-compliant facility, each batch undergoes stringent quality assurance to meet the demands of cutting-edge research and clinical applications. With excellent proliferative capacity and low immunogenicity, these cells are a trusted choice for scientists and clinicians worldwide.

Stem Cell Storage Temperatures

1. Cryopreserved (Long-Term Use) Temperature: –150°C to –196°C

Storage Method: Liquid nitrogen (LN₂) vapor phase or liquid phase

Why: At this temperature, all metabolic activity stops, and cells can be preserved for years to decades without loss of function

Storing stem cells at warmer temperatures—even briefly—can reduce viability and compromise cell membrane integrity.

2. Short-Term Holding (Before Use or Shipping) Temperature: –80°C

Storage Method: Ultra-low mechanical freezer Why: Acceptable for short-term storage (days to a few weeks) for prepared therapeutic doses or ready-to-thaw clinical vials

Stem Nova Network, for example, ships stem cells frozen on dry ice (approx. –78.5°C) for transit, with the expectation that they are transferred to –80°C or colder immediately upon receipt.

Not Suitable

Temperature      Reason Not Suitable
–20°C                 Causes ice crystal formation → cell death
0–4°C                 Cells begin to degrade; not viable for storage
Room Temp       Cells rapidly degrade in minutes to hours

Thawing Cells: Step-by-Step Procedure

1. Preparation of Warm Water
Warm sterile water in a 37°C water bath. Ensure that the water bath is calibrated correctly to maintain this temperature throughout the thawing process.

2. Surface Sterilization
Before handling the cell vial, wipe the outside with 70% ethanol or isopropanol to minimize contamination risks. This step is crucial for maintaining aseptic conditions.

3. Pressure Relief in Vial
In a biosafety cabinet, carefully twist the cap of the vial a quarter-turn to relieve any internal pressure that may have built up during freezing. Retighten the cap to prevent contamination.

4. Thawing Cells
- Place the vial in the 37°C water bath and gently shake it to facilitate thawing. Do not submerge the vial completely; only the lower part should be in contact with the warm water.
Monitor closely and remove the vial when only a small frozen cell pellet remains visible
(Thaw for NOT LONGER than 1 minute – to ice ball stage). Handle gently and completely avoid vortexing or shaking vigorously, as this will likely damage cells.

5. Second Surface Sterilization
Again, wipe the outside of the vial with 70% ethanol or isopropanol after thawing to ensure that any potential contaminants are removed before opening.

6. Dilution of Cells
Dilute the thawed cells by adding an equal volume of warmed culture medium (1:10 dilution). This step helps to reduce cellular stress and promotes recovery.

7. Centrifugation
Centrifuge the diluted cell suspension at 1200 RPM for 5 minutes max at room temperature (15 - 25°C). This process helps to pellet the cells while removing any cryoprotectant (e.g., DMSO) that may be present in the medium.

8. Supernatant Removal
After centrifugation, carefully remove the supernatant using a pipette. Leave a small amount of medium above the cell pellet to avoid disturbing it during resuspension.

9. Resuspension of Cell Pellet
Gently resuspend the cell pellet by flicking the tube lightly with your finger or using a pipette tip to mix very gently; Add 1 ml of solution; wait 1 min, slowly add another 2 ml of solution. Avoid vigorous mixing or vortexing, which could damage cells.

10. Final Medium Addition
After waiting another minute; slowly add up to 10 ml of the remaining warmed medium to the tube containing the resuspended cells. This gradual addition helpsto acclimate the cells back into their growth environment.

Ordering Information

Elevate your research or therapeutic development with our hUCT-MSCs. Available for immediate shipment, with comprehensive technical support included. Contact our team for bulk pricing, custom formats, or additional characterization data to suit your specific needs.

Unlock the potential of regenerative medicine with our Human Umbilical Cord Tissue-Derived Mesenchymal Stem Cells—your foundation for groundbreaking discoveries.

FDA Compliance Information

•  Cosmetic Classification: This product is a cosmetic under the Federal Food, Drug, and Cosmetic Act (21 U.S.C. § 301 et seq.) and is not a drug, biologic, or medical device. It is intended to enhance skin appearance (e.g., hydration, smoothness) and does not treat medical conditions or alter skin structure/function.
•  Labeling: Ingredients are listed per INCI standards (21 CFR 701).
•  Safety: Manufactured under cosmetic Good Manufacturing Practices (GMP, ISO 22716-aligned). Testing confirms sterility (no growth), no mycoplasma, and low endotoxin (<0.005 EU/mL).

For shipping policy please visit Our Shipping Policy Page.